ANTIBODIES

Contributor Information
- Name Wolf Reik
- Institute Babraham Institute
Tool Details
- Tool name: Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb
- Clone: AB3/63.3
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: All
- Host: Rat
- Application: IF ; IP ; DB
- Description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
- Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
- Isotype: IgG2a kappa
- Research area: Genetics; Stem cell biology
- For Research Use Only
Target Details
- Target: 5-Hydroxymethylcytosine (5hmC)
- Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Application Details
- Application: IF ; IP ; DB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -20°C
- Shipping conditions: Shipping at 4°C
Related Tools
References
- • Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.
- • Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.
- • Alterations of epigenetic signatures in hepatocyte nuclear factor 4a deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
- • Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.
- • Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.
- • Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
- • Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
- • 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.