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Contributor Information

  • Name Ciaran Morrison
  • Institute National University of Ireland, Galway; Centre For Chromosome Biology

Tool Details

  • Tool name: Anti-Centrobin [6D4F4]
  • Alternate names: Centrosomal BRCA2-interacting protein, LYST-interacting protein 8
  • Clone: [6D4F4]
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 100 kDa (runs at c. 110 kDa on WB)
  • Application: IHC ; IF ; IP ; WB
  • Description: Centrobin is a centriole-associated protein that asymmetrically localises to the daughter centriole, and is required for centriole duplication and cytokinesis.
  • Immunogen: Recombinant human centrobin amino acids 113-361 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Centrobin, UniProt ID:Q8N137
  • Target molecular weight: 100 kDa (runs at c. 110 kDa on WB)
  • Target background: Centrobin is a centriole-associated protein that asymmetrically localises to the daughter centriole, and is required for centriole duplication and cytokinesis.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Ogungbenro et al. 2018. J Cell Biol. 217(4):1205-1215. PMID: 29440264.
  • •  Centrobin controls primary ciliogenesis in vertebrates.