ANTIBODIES
Contributor Information
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti-Leg1 [1G1]
- Clone: 1G1
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Zebrafish
- Host: Mouse
- Application: IF
- Description: Leg1 is a novel protein of 361 amino acids. Preliminary data suggested that Leg1 (previously named as D12) is involved in early liver development. However, no detailed characterization of Leg1 has been reported thus far. There are two copies of leg1 in zebrafish, namely leg1a and leg1b. Both leg1a and leg1b are expressed in the larvae and adult liver with leg1a being the predominant form. Knockdown of Leg1a or Leg1b by their respective morpholinos specifically targeting their 59-UTR each resulted in a small liver phenotype, demonstrating that both Leg1a and Leg1b are important for early liver development.
- Immunogen: GST-Leg 1 fusion protein
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Liver-enriched gene 1 (previously called D12)
- Target background: Leg1 is a novel protein of 361 amino acids. Preliminary data suggested that Leg1 (previously named as D12) is involved in early liver development. However, no detailed characterization of Leg1 has been reported thus far. There are two copies of leg1 in zebrafish, namely leg1a and leg1b. Both leg1a and leg1b are expressed in the larvae and adult liver with leg1a being the predominant form. Knockdown of Leg1a or Leg1b by their respective morpholinos specifically targeting their 59-UTR each resulted in a small liver phenotype, demonstrating that both Leg1a and Leg1b are important for early liver development.
Application Details
- Application: IF
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Chang et al. 2011. PLoS One. 6(8):e22910. PMID: 21857963
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