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Contributor Information

  • Name Peter Beverley
  • Institute Cancer Research, UK London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CD3 recombinant antibody [UCH-T1™]
  • Clone: rUCH-T1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; WB
  • Description: Recombinant version of CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia’s. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains ( CD3?, CD3? and 2X CD3?), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. This is a recombinant version of anti-CD3 UCH-T1 . UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, SĂŠzary leukaemia’s and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.
  • Immunogen: Human infant thymocytes and Sezary cells.
  • Immunogen UniProt ID: P04234
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction; Immunology; Stem cell biology
  • Additional notes: UCH-T1Ě˝ is a registered trademark of Cancer Research Technology, Limited. All rights reserved.

  • For Research Use Only

Target Details

  • Target: CD3
  • Target background: Recombinant version of CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia's.Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains ( CD3?, CD3d and 2X CD3), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction.This is a recombinant version of anti-CD3 UCH-T1. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia's. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Szary leukaemia's and approximately 70% of acute lymphoblastic leukaemia's of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events.This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.

Application Details

  • Application: FACS ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS only
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Original hybridoma first published in: Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
  • •  Distinctive Fn characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.