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Contributor Information

  • Name Helen Turley
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-ATRX [39f]
  • Alternate names: RAD54, XNP
  • Clone: 39f
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Application: IHC ; IF ; WB
  • Description: Monoclonal antibody which binds N terminus of human ATRX.
  • Immunogen: Recombinant Protein
  • Immunogen UniProt ID: P46100
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: ATRX
  • Target background: ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres. This antibody binds to an epitope between amino acid residues 85-319 in the N-terminal half of human ATRX.

Application Details

  • Application: IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.
  • •  Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
  • •  Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
  • •  Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.
  • •  Fn significance of mutations in the Snf2 domain of ATRX.
  • •  Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.
  • •  Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
  • •  McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
  • •  Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.