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Contributor Information

  • Institute University of Cambridge

Tool Details

  • Tool name: Anti-HPV16 E1/E4 [TVG 403]
  • Clone: TVG 403
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human papilloma virus
  • Host: Mouse
  • Cancer type: Gynaecologic cancer
  • Molecular weight of the target: 10 kDa
  • Application: ELISA ; FACS ; IHC
  • Description: TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.
  • Immunogen: Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.
  • Isotype: IgG1
  • Research area: Cancer; Microbiology
  • Myeloma used: NS0

  • For Research Use Only

Target Details

  • Target: HPV-16 E1/E4 (early proteins E1 and E4).
  • Target molecular weight: 10 kDa
  • Target background: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer case. E1 and E2 are proteins involved in the regulation of viral DNA replication.

Application Details

  • Application: ELISA ; FACS ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Doorbar et al. 2000. J Virol. 74(21):10081-95. PMID: 11024137.
  •   The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.
  •   Doorbar et al. 1992. Virology. 187(1):353-9. PMID: 1371027.
  •   Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.