ANTIBODIES
Contributor Information
- Name Jenny Southgate
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-Keratin [LDS103]
- Clone: LDS103
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IF
- Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 17 and 18 are type I cytokeratins. Keratin 17 is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It has been shown to interact with CCDC85B. Keratin 18 is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Keratin 18 has been shown to interact with Collagen, type XVII, alpha 1, DNAJB6, Pinin and TRADD.
- Immunogen: Cytoskeletons of the human urothelial RT4 cell line.
- Isotype: IgG1
- Research area: Cell signaling and signal transduction
- For Research Use Only
Target Details
- Target: Keratin expressed in basal cells, pan-epithelial
- Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 17 and 18 are type I cytokeratins. Keratin 17 is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It has been shown to interact with CCDC85B. Keratin 18 is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Keratin 18 has been shown to interact with Collagen, type XVII, alpha 1, DNAJB6, Pinin and TRADD.
Application Details
- Application: IHC ; IF
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
References
- • Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.
- • Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.
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