ANTIBODIES

Contributor Information
- Name Ayham Alnabulsi
- Institute Vertebrate Antibodies Limited
Tool Details
- Tool name: Anti-a-MMP9 [4A3]
- Clone: 4A3
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human ; Rat
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Strain: Balb/c
- Description: Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis
- Immunogen: Ovalbumin-conjugated synthetic peptide; acetylated-FQTFEGDLK
- Isotype: IgG1 kappa
- Research area: Cell biology; Cancer; Tissue-specific biology; Cell signaling and signal transduction; Biochemistry
- Myeloma used: P3X63Ag8.653
- For Research Use Only
Target Details
- Target: Activated MMP9 (matrix metalloproteinase 9)
- Target background: Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis
Application Details
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
References
- • Cirella et al. 2016. Thesis
- • Goktolga et al. 2015. Exp Ther Med. 9(5):1813-1818. PMID: 26136898.
- • Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model.
- • Duncan et al. 1998. Eur J Biochem. 258(1):37-43. PMID: 9851689.
- • Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.