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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]
  • Clone: FIS 2H9/1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Molecular weight of the target: 170 kDa
  • Application: IHC ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: This antibody is a useful marker of apicobasal polarity in the epithelium.
  • Immunogen: Lysate of zebrafish intestine
  • Isotype: IgG1
  • Research area: Cell biology; Developmental biology; Tags and cell markers
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Zebrafish Basolateral Pole of Cells
  • Target molecular weight: 170 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 2H9 recognises a membrane-associated epitope on the gut epithelial cells, concentrated basolaterally. In one month old zebrafish it appears especially prominent in the basal parts of the elongated cells of the villi.

Application Details

  • Application: IHC ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  • •  Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.