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Contributor Information

  • Name Roy Bicknell
  • Institute Absolute Antibody ; University of Oxford

Tool Details

  • Tool name: Anti-VEGF [VG76e]
  • Clone: rVG76e
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Cancer type: Neurological cancer; Liver cancer; Gynaecologic cancer; Genitourinary cancer; Genitourinary cancer; Breast cancer
  • Molecular weight of the target: 38-44 kDa
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Recombinant monoclonal antibody with use investigating MVCD1, angiogenesis and various cancers.
  • Immunogen: Human VEGF189 expressed in E. coli.
  • Immunogen UniProt ID: P15692
  • Isotype: IgG1
  • Research area: Cancer; Tissue-specific biology; Cell signaling and signal transduction; Metabolism

  • For Research Use Only

Target Details

  • Target: Vascular Endothelial Growth Factor (VEGF)
  • Target molecular weight: 38-44 kDa
  • Target background: VEGF is a 34-43 kD polypeptide growth factor, part of the PDGF family. There are 7 homo-dimeric isoforms (A-G) with VEGF 121, VEGF 165 and VEGF189 being the most extensively studied (F,D and B respectively). They are generated by alternative splicing and binding to VEGF receptors (FLT-1 and KDR) which are selectively expressed on vascular endothelial cells. VEGF elicits mitogenic effects on endothelial cells and is strongly angiogenic, with a role in cancer and metastasis. VEGF is involved in vasculogenesis and endothelial growth. VEGF expression is potentiated, and the protein is secreted by tumour cells in response to hypoxia, by activated oncogenes, growth factors, nitric oxide and a variety of cytokines. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g. glioma, HCC, ovarian, bladder, prostate, breast cancer etc. VEGF is an angiogenic growth factor and a prognostic indicator for cancer when detected in serum. This is a recombinant version of the anti-VEGF monoclonal antibody. This antibody binds VEGF-A.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS only
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Original hybridoma first published in Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.
  • •  A monoclonal antibody to the human epidermal growth factor receptor.