ANTIBODIES
Contributor Information
- Name Yee Joo Tan
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti Nucleocapsid Protein of MERS CoV [7H6]
- Alternate names: Anti-Nucleocapsid Protein of MERS CoV [7H6]
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mouse
- Host: Mouse
- Application: ELISA ; IF ; IP ; WB
- Strain: Balb/c
- Description: Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus. Background and Research Application The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus. One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs). However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses. The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
- Immunogen: R9UM87
- Immunogen UniProt ID: R9UM87
- Isotype: IgG2b kappa
- Research area: Apoptosis and autophagy; Cell biology; Microbiology
- For Research Use Only
Target Details
- Target: Nucleocapsid Protein of MERS-CoV
- Target background: Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus. Background and Research Application The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus. One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs). However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses. The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
Application Details
- Application: ELISA ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Aboagye et al. 2018. Biosci Rep. 38(5):. PMID: 30242057.
- • Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.
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