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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
  • Clone: FIS 4E8/1; 2F11
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Cancer type: Digestive / Gastrointestinal cancer
  • Molecular weight of the target: 200 kDa
  • Application: IHC ; IP ; WB
  • Strain: Balb/c
  • Description: This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
  • Immunogen: Lysate of zebrafish intestines
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Tags and cell markers; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Zebrafish gut absorptive cell epitopes
  • Target molecular weight: 200 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli).

Application Details

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
  • •  Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
  • •  Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.
  • •  Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.
  • •  Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.
  • •  Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
  • •  Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  • •  Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.