ANTIBODIES
Contributor Information
- Name Keith Willison
- Institute The Institute of Cancer Research
Tool Details
- Tool name: Anti-TCP1 [22b]
- Clone: 22b
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Chicken ; Dog ; Hamster ; Mouse ; Rat
- Host: Rat
- Application: ELISA ; IP ; WB
- Description: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.
- Immunogen: C-terminal half of the full length murine TCP protein
- Isotype: IgG2a
- Research area: Apoptosis and autophagy; Cancer; Cell biology
- Myeloma used: Y3.AG.1.2.3
- For Research Use Only
Target Details
- Target: T-complex polypeptide-1 (TCP1; CCTalpha)
- Target background: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.
Application Details
- Application: ELISA ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Zhu H et al, 2010. Proteomics (19):3480-93 PMID 20815088
- • Harrison-Lavoie et al. 1993. EMBO J. 12(7):2847-53. PMID: 8335000.
- • A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins.
- • Willison et al. 1989. Cell. 57(4):621-32. PMID: 2655925.
- • The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes.
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