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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-LewisX [29]
  • Alternate names: Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
  • Clone: 29
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS
  • Description: Monoclonal antibody directed at against Lewis X antigen, with use in differentiating between leukaemia's and aids in the purification of stem cells.
  • Immunogen: Fibronectin purified human monocytes .
  • Immunogen UniProt ID: P22083
  • Isotype: IgM
  • Research area: Cancer; Immunology

  • For Research Use Only

Target Details

  • Target: Lewis X (CD15)
  • Target background: Lewis X (CD15) is a branched pentasaccharide found on glycolipids, glycoproteins and proteoglycans of cells of gastric mucosa, nervous system, neutrophils, eosinophils, and monocytes. Lewis X is distributed abnormally in myeloid leukaemia's and is commonly used in the diagnosis of Hodgkin's disease. Lewis X is believed to enhance cell adhesion, either directly or by promoting integrin activation. Cells with high surface expression of Lewis X antigen, display strong self-aggregation, based on calcium-dependent Lewis X-Lewis X interaction. Anti-Lewis X (29) is useful for leukaemia typing and the purification of stem cells.

Application Details

  • Application: FACS

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Bernstein I & Self S. Report of the myeloid section of the Second International Workshop on Leucocyte Typing II, Human leucocyte Differentiation Antigens. Published by Springer-Verlag, New York. 1986.