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Contributor Information

  • Name Helle Ulrich
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-PCNA [5E6/2]
  • Clone: 5E6/2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Saccharomyces cerevisiae
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 30 kDa
  • Application: WB ; ELISA ; IP ; WB
  • Strain: CD1
  • Description: Monoclonal antibody which binds PCNA, a tumour proliferation marker.
  • Immunogen: Purified recombinant His6-POL30 (PCNA) from budding yest, Saccharomyces cerevisiae, produced in Escherichia coli
  • Immunogen UniProt ID: P12004
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.
  • Target molecular weight: 30 kDa
  • Target background: Monoclonal antibody which binds PCNA, a tumour proliferation marker. Background and Research Application PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It is required for cellular DNA synthesis, and in vitro replication of SV40 DNA. It helps to co-ordinate the leading and lagging strand synthesis at the replication fork. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is the replicative clamp protein, an essential processivity factor for DNA polymerases and an interaction partner for many other proteins. This protein is potentially a therapeutic target in cancer therapy and a useful marker for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). This antibody recognises endogenous PCNA protein from budding yeast, Sacchoromyces cerevisiae.

Application Details

  • Application: WB ; ELISA ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Paul Solomon Devakumar et al. 2019. Nucleic Acids Res. 47(13):6826-6841. PMID: 31114918.
  • •  Schalbetter et al. 2015. Proc Natl Acad Sci U S A. 112(33):E4565-70. PMID: 26240319.
  • •  Kubota et al. 2011. Mol Cell Proteomics. 10(7):M110.005561. PMID: 21505101.
  • •  Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint.
  • •  Parker et al. 2008. EMBO J. 27(18):2422-31. PMID: 18701921.
  • •  SUMO modification of PCNA is controlled by DNA.
  • •  Davies et al. 2008. Mol Cell. 29(5):625-36. PMID: 18342608.
  • •  Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a.
  • •  Windecker et al. 2008. J Mol Biol. 376(1):221-31. PMID: 18155241.
  • •  Architecture and assembly of poly-SUMO chains on PCNA in Saccharomyces cerevisiae.