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Contributor Information

  • Name Roy Jefferis
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-Integrin a4 [BU49]
  • Alternate names: Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; Human Platelet Alloantigen System 5; Platelet Glycoprotein GPIa; Platelet Antigen Br; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR
  • Clone: BU49
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 150 kDa
  • Application: FACS ; IP
  • Description: BU49 induces phosphorylation of a cAMP response element-binding protein (CREB) in the human monocyte-like cell line, U937.
  • Immunogen: Hicett myeloma cell line
  • Isotype: IgG1
  • Research area: Cell biology; Tissue-specific biology; Cell signaling and signal transduction; Immunology; Stem cell biology

  • For Research Use Only

Target Details

  • Target: Integrin alpha 4 (CD49d)
  • Target molecular weight: 150 kDa
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha 4 (CD49d) is a 150 kDa protein characterised by a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain. Hetrodimers are formed with integrin beta 1 and 7.

Application Details

  • Application: FACS ; IP

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Ikewaki et al. 2002. Microbiol Immunol. 46(10):685-95. PMID: 12477247.
  • •  A very late activating antigen-alpha4 (CD49d) monoclonal antibody, BU49 induces phosphorylation of a cAMP response element-binding protein (CREB), resulting in induction of homotypic cell aggregation and enhancement of interleukin-8 (IL-8) production.
  • •  Nojima et al. 1992. J Exp Med. 175(4):1045-53. PMID: 1372641.
  • •  Ligation of VLA-4 on T cells stimulates tyrosine phosphorylation of a 105-kD protein.