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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Zebrafish gut secretory cell epitopes [FIS 6G5/1]
  • Clone: FIS 6G5/1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155-2158).
  • Immunogen: Lysate of zebrafish intestine
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Tags and cell markers; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Zebrafish gut secretory cell epitopes
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155-2158).

Application Details

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
  • •  Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
  • •  Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.
  • •  Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.
  • •  Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.
  • •  Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
  • •  Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  • •  Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.