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Contributor Information

  • Name Jacqueline Cordell
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-Leu2 [C8/144B] mAb
  • Alternate names: CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen; Alpha Polypeptide (P32); MAL; T-Cell Surface Glycoprotein CD8 Alpha Chain; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T8 T-Cell Antigen; CD8a Antigen; Leu2; P32
  • Clone: C8/144B
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Blood cancer
  • Application: IHC ; IF ; IP ; WB
  • Description: C8/144B can be used to detect these cells in tissue sections and also their neoplastic counterparts.
  • Immunogen: Synthetic peptide (the 13 C-terminal residues of the cytoplasmic domain of human CD8a)
  • Isotype: IgG1
  • Research area: Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Leu2 (CD8)
  • Target background: CD8 is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. CD8 is present on human suppressor/cytotoxic T cells which make up 30% of circulating T cells.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  van Gool et al. 2015. Clin Cancer Res. 21(14):3347-55. PMID: 25878334.
  • •  POLE Proofreading Mutations Elicit an Antitumor Immune Response in Endometrial Cancer.
  • •  Harlin et al. 2009. Cancer Res. 69(7):3077-85. PMID: 19293190.
  • •  Chemokine expression in melanoma metastases associated with CD8+ T-cell recruitment.
  • •  Sato et al. 2005. Proc Natl Acad Sci U S A. 102(51):18538-43. PMID: 16344461.
  • •  Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer.
  • •  Mason et al. 1992. J Clin Pathol. 45(12):1084-8. PMID: 1479035.
  • •  Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.