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Contributor Information

  • Name Karen Pulford
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-Haemoglobin Scavenger Receptor [GHI/61]
  • Clone: GHI/61
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; Fn ; WB
  • Description: Monoclonal antibody which recognises circulating blood monocytes and the large majority of tissue macrophages, without labelling granulocytes or lymphocytes.
  • Immunogen: Hairy cell leukaemia cells
  • Immunogen UniProt ID: Q86VB7
  • Isotype: IgG1
  • Research area: Tissue-specific biology; Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Haemoglobin Scavenger Receptor (M130; CD163)
  • Target background: Anti-haemoglobin scavenger receptor labels the majority of monocytes and tissue macrophages. It recognises a membrane glycoprotein with molecular weight 130,000 (reduced) and 110,000 (unreduced). The glycoprotein antigen is expressed exclusively on the cell surface of human monocytes and macrophages, which evolves predominantly in the late phase of inflammation. The glycoprotein GHI/61 binds to CD163, a transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It is a receptor for the haemoglobin-haptoglobin complex. CD163 expression on monocytes and macrophages changes as these cells differentiate, as such GHI/61 can detect levels of differentiated monocytes and macrophages. CD163 has high affinity for the haemoglobin-haptoglobin complex and upon ligand binding, leads to the production of IL-6 and IL-10. GHI/61 monoclonal antibody was created to label monocytes and macrophage with increased specificity, given similar antibodies frequently label lymphocytes etc., or only recognise a particular subset of macrophages.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Salerno-Goncalves et al. 2019. PLoS Negl Trop Dis. 13(8):e0007650. PMID: 31412039.
  • •  Amini et al. 2019. BMC Cancer. 19(1):316. PMID: 30953461.
  • •  Schulz et al. 2019. Sci Rep. 9(1):1925. PMID: 30760760.
  • •  Silva et al. 2019. Front Immunol. 10:1508. PMID: 31333653.
  • •  Gruenbacher et al. 2019. Front Immunol. 10:1870. PMID: 31447857.
  • •  Moura et al. 2012. Eur J Immunol. 42(11):2925-36. PMID: 22851198.
  • •  CD163 favors Mycobacterium leprae survival and persistence by promoting anti-inflammatory pathways in lepromatous macrophages.
  • •  Moniuszko et al. 2006. Clin Vaccine Immunol. 13(6):704-7. PMID: 16760331.
  • •  Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants.
  • •  Mller et al. 2002. Blood. 99(1):378-80. PMID: 11756196.
  • •  Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma.
  • •  Law et al. 1993. Eur J Immunol. 23(9):2320-5. PMID: 8370408.
  • •  A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.
  • •  Pulford et al. 1992. Immunology. 75(4):588-95. PMID: 1592433.
  • •  A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.