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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CHD4 [CHD4 3F2/4]
  • Clone: CHD4 3F2/4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse ; Human
  • Host: Mouse
  • Molecular weight of the target:
  • Application: ChIP ; ELISA ; FACS ; IF ; IHC ; IP ; WB
  • Strain: CD1
  • Description: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
  • Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
  • Isotype: IgG2a
  • Research area: Genetics; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Chromodomain-helicase-DNA-binding protein 4 (CHD4/Mi2b)
  • Target molecular weight:
  • Target background: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Application Details

  • Application: ChIP ; ELISA ; FACS ; IF ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -80°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Sparmann et al. 2013. EMBO J. 32(11):1598-612. PMID: 23624931.
  • •  The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation.
  • •  Curtis et al. 2012. Mol Cell Biol. 32(7):1312-20. PMID: 22290435.
  • •  The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling.
  • •  Ramrez et al. 2009. Epigenetics. 4(8):532-6. PMID: 19923891.
  • •  The Mi-2/NuRD complex: a critical epigenetic regulator of hematopoietic development, differentiation and cancer.