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Contributor Information

  • Name Jesper Svejstrup
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
  • Primary citation Kristjuhan et al. 2002. Mol Cell. 10(4):925-933. PMID: 12419235.

Tool Details

  • Tool name: Anti-RNA polymerase II [CTD 4H8] mAb
  • Alternate names: RNAPII antibody; 4H8 antibody; CTD 4H8 antibody
  • Clone: CTD 4H8
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Saccharomyces cerevisiae
  • Host: Mouse
  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
  • Strain: Balb/c
  • Description: Anti-RNA polymerase II (RNAPII), clone CTD 4H8 is mouse monoclonal antibody. CTD 4H8 recognises both hypo- and hyperphosphorylated RNAPII. CTD 4H8 is known to be reactive with the C-terminal domain (CTD) in humans and saccharomyces cerevisiae.

    RNAPII is a multi-subunit enzyme responsible for the transcription of protein-coding genes. The C-terminal domain (CTD) is a highly conserved domain unqiue to RNAPII, coordinating factors that regulate transcription initiation, elongation and mRNA processing. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation.

    The immunogen is a peptide containing 10 repeats of synthetic peptide YSPTSPS using chemically synthesised phospho-Ser5.

    CTD 4H8 can be used in chromatin immunoprecipitation (ChIP) assays.
  • Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
  • Isotype: IgG1
  • Research area: Epigenetics & Nuclear Signalling
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: RNA Polymerase II
  • Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

Application Details

  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Williamson et al. 2017. Cell. 168(5):843-855. PMID: 28215706.
  • •  Pang et al. 2014. Oncogene. 33(31):4039-4049. PMID: 24141769.
  • •  Seo et al. 2014. Nucleic Acids Res. 42(2):1016-25. PMID: 24163101.
  • •  Keren-Shaul et al. 2013. PLoS One. 8(1):e53506. PMID: 23326444.
  • •  Harrigan et al. 2011. J Cell Biol. 193(1):97-108. PMID: 21444690.
  • •  Li et al. 2006. Mol Biol Cell. 17(9):3848-3859. PMID: 16807355.
  • •  Yart et al. 2005. Mol Cell Biol. 25(12):5052-5060. PMID: 15923622.
  • •  Engelhardt et al. 2005. J Virol. 79(9):5812-5818. PMID: 15827195.
  • •  Kristjuhan et al. 2003. Proc Natl Acad Sci U S A. 100(13):7551-7556. PMID: 12796514.
  • •  Kristjuhan et al. 2002. Mol Cell. 10(4):925-933. PMID: 12419235.