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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-PSMA5 [AH1.1]
  • Clone: AH1.1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Xenopus laevis
  • Host: Mouse
  • Application: FACS ; IHC ; IP ; WB
  • Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.
  • Immunogen: Sub-unit 5a
  • Isotype: IgG1
  • Research area: Cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Proteasome complex; subunit 5a (PSMA5)
  • Target background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.

Application Details

  • Application: FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Liu et al. 2014. J Clin Invest. 124(5):2059-70. PMID: 24691443.
  • •  Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
  • •  Kley et al. 2012. Brain. 135(Pt 9):2642-60. PMID: 22961544.
  • •  Pathophysiology of protein aggregation and extended phenotyping in filaminopathy.
  • •  Yi et al. 2010. Cell Tissue Res. 341(2):325-40. PMID: 20526895.
  • •  Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization.
  • •  Arlt et al. 2009. Oncogene. 28(45):3983-96. PMID: 19734940.
  • •  Increased proteasome subunit protein expression and proteasome activity in colon cancer relate to an enhanced activation of nuclear factor E2-related factor 2 (Nrf2).