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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Cdc27 [AF 3.1]
  • Alternate names: Cell Division Cycle 27; Anaphase Promoting Complex Subunit; DS143E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2
  • Clone: AF 3.1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mammalian ; Xenopus laevis
  • Host: Mouse
  • Molecular weight of the target: 80 kDa
  • Application: ELISA ; FACS ; IHC ; IP ; WB
  • Strain: Balb/c
  • Description: AF 3.1 is recommended for the immunoprecipitation of active APC.
  • Immunogen: A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
  • Isotype: IgG2b
  • Research area: Cell biology; Biochemistry
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Cell division cycle protein 27 (cdc27)
  • Target molecular weight: 80 kDa
  • Target background: Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis.

Application Details

  • Application: ELISA ; FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Mateo F et al. Oncogene. 2009 Jul 23
  • •  28(29):2654-66.
  • •  Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.
  • •  Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
  • •  Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.
  • •  Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
  • •  Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.
  • •  An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
  • •  Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.
  • •  The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.