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Contributor Information

  • Name Irene Leigh
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Keratin5 & 14 [LH8]
  • Clone: LH8
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IHC ; IF
  • Strain: Balb/c
  • Description: LH8 may be useful as a basal cell marker and myoepithelial marker in stratified squamous or mixed epithelia.
  • Immunogen: The 50,000 Mr band (including K14) was cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
  • Isotype: IgM
  • Research area: Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Keratin 5 & 14
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 5 and 14 are the predominant keratins in basal layer keratinocytes in stratified epithelia. Their expression is restricted to basal cells and are found on all keratinocytes. Keratin 14 is one of two keratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Mutations in keratin 5 or 14 cause the skin blistering disorder epidermolysis bullosa simplex.

Application Details

  • Application: IHC ; IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Moser et al. 2014. PLoS One. 9(4):e94118. PMID: 24705787.
  •   Prince et al. 2007. Proc Natl Acad Sci U S A. 104(3):973-8. PMID: 17210912.
  •   Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.
  •   de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.
  •   Keratin expression in discoid lupus erythematosus.