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Contributor Information

  • Name Walter Bodmer
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-HLADR beta chain 1 [DA2]
  • Clone: DA2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing.
  • Immunogen: The membranes of LKT cells (a B lymphoblastoid cell line)
  • Immunogen UniProt ID: P01911
  • Isotype: IgG1
  • Research area: Immunology
  • Myeloma used: P3X63Ag8

  • For Research Use Only

Target Details

  • Target: Human Leukocyte Antigen DR ? chain 1 (1st domain, monomorphic, MHC class II)
  • Target background: Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing. Background and Research Application Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response. DA2 may be used for tissue typing. This antibody will bind all DR and DP antigens except DR7, and can aid with HLA typing.

Application Details

  • Application: ELISA ; FACS ; IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.
  • •  Monoclonal antibodies to HLA--DRw determinants.
  • •  Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
  • •  Monoclonal antibodies for analysis of the HLA system.