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Contributor Information

  • Name Irene Leigh
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Keratin14 [LL001]
  • Clone: LL001
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse ; Pig
  • Host: Mouse
  • Molecular weight of the target: 50 kDa
  • Application: IHC ; IF ; Ip ; WB
  • Strain: Balb/c
  • Description: Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types.
  • Immunogen: Peptide (last 16 carboxy-terminal amino acids of human keratin) coupled to thyroglobulin by the extra Cys added at the N terminus.
  • Isotype: IgG2a kappa
  • Research area: Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Keratin 14
  • Target molecular weight: 50 kDa
  • Target background: Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types.

Application Details

  • Application: IHC ; IF ; Ip ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Sun et al. 2011. Endocrinology. 152(8):3233-45. PMID: 21628386.
  • •  Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
  • •  Karashima et al. 2002. J Cell Sci. 115(Pt 24):5027-37. PMID: 12432088.
  • •  Interaction of periplakin and envoplakin with intermediate filaments.
  • •  D'Alessandro et al. 2002. J Cell Sci. 115(Pt 22):4341-51. PMID: 12376565.
  • •  Keratin mutations of epidermolysis bullosa simplex alter the kinetics of stress response to osmotic shock.
  • •  Purkis et al. 1990. J Cell Sci. 97 ( Pt 1):39-50. PMID: 1701769.
  • •  Antibody markers of basal cells in complex epithelia.
  • •  Leigh et al. 1988. J Invest Dermatol. 90(5):639-42. PMID: 3283248.
  • •  Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.