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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-p21 [AC8.3]
  • Alternate names: Cyclin-Dependent Kinase Inhibitor 1A; CDK-Interacting Protein 1; CDKN1; CAP2; MDA-6; CIP1; SDI1; WAF1; P21; Melanoma Differentiation Associated Protein 6; Wild-Type P53-Activated Fragment 1; CDK-Interaction Protein 1; DNA Synthesis Inhibitor; P21CIP1; MDA6; PIC1
  • Clone: AC8.3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
  • Description: AC8.3 can be used as a detection system for rapidly dividing cells and is recommended for use in tissue culture.
  • Immunogen: His-tagged human p21 expressed in bacleva virus.
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: p21
  • Target background: Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and overexpression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Application Details

  • Application: ELISA ; FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Xu et al. 2010. Biol Pharm Bull. 33(5):743-51. PMID: 20460749.
  • •  Targeting the Na(+)/K(+)-ATPase alpha1 subunit of hepatoma HepG2 cell line to induce apoptosis and cell cycle arresting.
  • •  Petrides et al. 2008. J Comp Neurol. 507(5):1653-62. PMID: 18241050.
  • •  Differential output of the high-sensitivity rod photoreceptor: AII amacrine pathway.