ANTIBODIES

Contributor Information
- Name Julian Gannon
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: Anti-CyclinA [E23.1]
- Alternate names: CCNA1; Cyclin A1; Testicular Tissue Protein Li 34; CT146
- Clone: E23.1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mammalian
- Host: Mouse
- Application: FACS ; IHC ; IF ; IP ; WB
- Description: E23.1 is extremely sensitive for immunoblotting Cyclin A.
- Immunogen: Bovine Cyclin A fusion protein.
- Isotype: IgG2a
- Research area: Cancer; Cell biology; Genetics
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Cyclin A
- Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
Application Details
- Application: FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
References
- • Hagen et al. 2013. Cell Div. 8(1):10. PMID: 23886499.
- • Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis.
- • Li et al. 2012. Proc Natl Acad Sci U S A. 109(31):12550-5. PMID: 22802651.
- • Gap 1 phase length and mouse embryonic stem cell self-renewal.
- • Hogg et al. 2011. Free Radic Biol Med. 50(9):1065-74. PMID: 21256959.
- • Effect of nitric oxide on neointimal hyperplasia based on sex and hormone status.
- • Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
- • Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
- • Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
- • Simian virus 40 large T antigen associates with cyclin A and p33cdk2.