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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Cdk1 [17 (A17)]
  • Alternate names: Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1
  • Clone: 17 (A17)
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Chicken ; Human ; Mouse ; Rat ; Xenopus laevis
  • Host: Mouse
  • Molecular weight of the target: 34 kDa
  • Application: ELISA ; IHC ; IP ; WB
  • Strain: Balb/c
  • Description: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
  • Immunogen: A carboxy-terminal fragment starting at methionine 85 of p34 cdc2 (Xenopus laevis).
  • Isotype: IgG2a
  • Research area: Cancer; Cell biology; Neurobiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Cyclin dependent kinase 1 (Cdk1, cdc2)
  • Target molecular weight: 34 kDa
  • Target background: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.

Application Details

  • Application: ELISA ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.
  • •  Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.
  • •  Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.
  • •  Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.
  • •  Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.
  • •  A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.
  • •  Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.
  • •  The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.
  • •  Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.
  • •  Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.
  • •  Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.
  • •  Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.