Product Image

Contributor Information

  • Name Maria E Teves Ph.D.
  • Institute Virginia Commonwealth University
  • Primary citation Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344

Tool Details

  • Tool name: Spag17/Tomato-EGFP/CMV-Cre mouse
  • Tool type: Mouse
  • Disease: Primary cilia dykinesia, accelerated aging, male infertility, and multi-organ fibrosis
  • Model: Knock-out
  • CRISPR: No
  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
  • Genetic background and cross history: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
  • Phenotype: Multi-organ fibrosis
  • Zygosity: Homozygous
  • Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
  • Description: This is a knockout mouse model for the global deletion of the Spag17 gene linked to a Tomato-EGFP system for cell linage tracing.
    This mouse model has the potential to generate a knockout for Spag17 when crossed with a Cre mouse. Cells will express an EGFP fluorophore for in vivo tracking if they are deficient in Spag17 and presents an avanue for studying primary cili dyskinesia, development, skeletal dysplasia, primary cilia disfunction, aging, infertility, and multi-organ fibrosis
  • Research area: Skeletal dysplasia; Cell biology; Primary cilia dyskinesia; Organ fibrosis
  • Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice demonstrated a mixed background of C57BL/6J–129S4/SvJ.

  • For Research Use Only

Target Details

Application Details

  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging

Handling

Documentation

Related Tools

Product Image

Inva3b1 Mouse

#151549
Product Image

Pik3ca-H1047R conditional Flp inducible

#158395
Product Image

FH1 KO Mouse

#151657

References

  • •  Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344
  • •  Teves et al. PLoS One 10(5) PMID: 26017218