EXPERIMENTAL MODELS
Contributor Information
- Name Fiona Watt
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: InvEE Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Conditional: Yes
- Conditional description: Conditional expression of MEK1 under involucrin promoter giving tissue-specific suprabasal epidermal expression.
- Genetic background and cross history: An involucrin - MEK1 transgene expression construct was injected into male pronucleus of fertilized F1 hybrid CBAxC57Bl/6 embryos. Transgene-positive mice were mated with wildtype F1 CBAxC57BL/6 to generate founder lines.
- Phenotype:
- Zygosity:
- Description: A transgenic mouse expressing constitutively active MEK 1 in the epidermis. The mouse displays many phenotypes associated with psoriasis including abnormal keratinocyte differentation, hyperproliferation of keratinocytes and infiltration of inflammatory elements. Disease model for psoriasis; in vivo study of constituitively active murine MEK1 mutant (S217E/S221E) in skin.
- Research area: Cell signaling and signal transduction; Genetics
- Production details: An involucrin - MEK1 transgene expression construct was injected into male pronucleus of fertilized F1 hybrid CBAxC57Bl/6 embryos. Transgene-positive mice were mated with wildtype F1 CBAxC57BL/6 to generate founder lines.
- Additional notes:
- For Research Use Only
Target Details
- Target: MEK1
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
References
- • Carroll et al. 1995. Cell. 83(6):957-68. PMID: 8521519.
- • Suprabasal integrin expression in the epidermis of transgenic mice results in developmental defects and a phenotype resembling psoriasis.
.svg)
Availability: 
.svg)
.svg)
