EXPERIMENTAL MODELS
Contributor Information
- Name David Elliott
- Institute Newcastle University
Tool Details
- Tool name: RBMXL2 conditional KO mouse
- Alternate names: RBMX Like 2, Testis-Specific Heterogeneous Nuclear Ribonucleoprotein G-T, RNA-Binding Motif Protein, X-Linked-Like-2, HNRNPGT
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Male infertility
- Model: Conditional KO
- Model description:
- Conditional: Yes
- Conditional description: Crossing with a Cre expressing mouse is required for knockdown
- Phenotype: Male KO mice had smaller testis devoid of sperm. No effect on female fertility
- Zygosity: Homozygous
- Strain: C57BL/6
- Description: DNA within a gene is transcribed to make molecules of ribonucleic acid (RNA). The cell then modifies many of these RNAs in a process called splicing before using them as templates to make proteins. The RBMXL2 protein is involved in pre-mRNA processing, metabolism and transport and is only made during and after meiosis in humans and most other mammals. RBMXL2 is primarily localized to the nuclei of meiotic spermatocytes and as such is a candidate gene for autosomal male infertility
- Research area: Cell biology
- Production details: A targeting construct in which the Rbmxl2 open reading frame was flanked by LoxP sites was made using standard molecular biology techniques, and electroporated into ES129 cells. Positive clones were injected into blastocysts to create chimaeras and bred to yield agouti pups heterozygous for the targeted locus. The original mice containing the Neomycin gene were crossed to FlpE mice to remove the Neo gene and to generate the Rbmxl2 LoxP conditional allele. Crossing with a Cre expressing mouse is required for knockdown
- For Research Use Only
Target Details
- Target: RBMXL2
Application Details
Handling
Documentation
Related Tools
References
- • Ehrmann et al. 2019. Elife. 8:. PMID: 30674417
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