Product Image

Contributor Information

  • Name Alfred Schinkel
  • Institute Netherlands Cancer Institute

Tool Details

  • Tool name: Oct1/2 Knock Out Mouse
  • Alternate names: SLC22A1/2, Solute Carrier Family 22 Member 1/2, Organic Cation Transporter 1/2
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Knock-Out
  • Conditional: No
  • Application: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.
  • Genetic background and cross history: The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations
  • Zygosity: Homozygous
  • Description: Oct1/2 are polyspecific organic cation transporters in the liver, kidney, intestine, and other organs. They are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.
  • Research area: Cancer; Cell signaling and signal transduction; Drug development
  • Production details: The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations
  • Breeding information: The colony is maintained by mating doubly homozygous mic
  • Additional notes: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations. The Oct1/2 mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute

  • For Research Use Only

Target Details

  • Target: Oct1 and Oct2

Application Details

  • Application: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

Related Tools

Product Image

MDR KO Mouse

#154094
Product Image

Endoglin Floxed Mouse

#151754
Product Image

JamC -/- Mouse

#151562

References

  • •  Jonker et al. 2003. Mol Cell Biol. 23(21):7902-8. PMID: 14560032.
  • •  Deficiency in the organic cation transporters 1 and 2 (Oct1/Oct2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations.
  • •  Jonker et al. 2001. Mol Cell Biol. 21(16):5471-7. PMID: 11463829.
  • •  Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene.