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Contributor Information

  • Name Ian Tomlinson
  • Institute University of Oxford

Tool Details

  • Tool name: Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse
  • Alternate names: IDH1, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Glioma
  • Model: Knock-In
  • Conditional: Yes
  • Phenotype: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen.
  • Strain: C57BL/6
  • Description: Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.
  • Research area: Cancer; Neurobiology
  • Additional notes: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen. The mice carry a tamoxifen-inducible Cre, Nestin-CreER(T2) which in adult mice targets Cre to the subventricular zone (SVZ) and the other major neurogenic niche, the subgranular zone (SGZ) of the hippocampal dentate gyrus. When induced using tamoxifen to express the Idh1R132H mutant protein the mice are referred to as Tam-Idh1-KI mice.

  • For Research Use Only

Target Details

  • Target: Isocitrate dehydrogenase 1

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.
  • •  Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.