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Contributor Information

  • Name Catrin Pritchard
  • Institute University of Leicester

Tool Details

  • Tool name: BRaf1 KI Mouse
  • Alternate names: LSL B-RafV6E
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Cancer type: Skin cancer; Digestive / Gastrointestinal cancer
  • Model: Knock-In
  • Conditional: Yes
  • Conditional description: B-Raf gene with loxP sites flanking wildtype exons 15 to 18 (plus STOP) inserted before mutant exons 15 (V600E) to 18, enabling conditional Cre-mediate excision of wildtype exons and simultaneous knockin of mutant exons.
  • Cell signalling pathway: Ras/Raf signalling
  • Genetic background and cross history: E14.1/C57BL6
  • Zygosity: Heterozygous
  • Description: Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways The LSL-B-Raf-V600E targeting vector, containing a loxP flanked minigene+polyA encoding wildtype exons 15 to 18, amd a loxP flanked resistance cassette, and a substitution of residue 600 (V to E) in the endogenous exon 15, was transfected into E14.1 ES cells. Properly targeted ES cells containing homologous recombination events were injected into C57BL6 balstocysts. Chimeric mice were backcrossed on the C57BL6 background.
  • Research area: Cancer; Cell signaling and signal transduction; Genetics

  • For Research Use Only

Target Details

  • Target: BRaf V600E (oncogenic mutant)

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Mercer et al. 2005. Cancer Res. 65(24):11493-500. PMID: 16357158.