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Contributor Information

  • Name Clive Dickson
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: FGFR2b Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Gastric
  • Conditional: Yes
  • Conditional description:
  • Genetic background and cross history: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
  • Phenotype:
  • Zygosity: Homozygous
  • Description: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
  • Research area: Cancer; Cell signaling and signal transduction; Genetics; Immunology
  • Production details: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
  • Additional notes:

  • For Research Use Only

Target Details

  • Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
  • •  The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
  • •  De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
  • •  An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.