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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MRP-14 (S100a9 -/-) Knockout Mouse
  • Alternate names: Myeloid-related protein 14 (MRP-14)
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Inflammatory disease;
  • Model: Knock-Out
  • Genetic background and cross history: The S100a9 gene was disrupted in ES cells with a replacement-type targeting vector and the strategy shown in the publication. ES cells were electroporated, and of 196 neomycin (G418)-resistant clones, 13 were correctly targeted. Two targeted clones were injected into C57BL/6 mouse blastocysts, and two independent strains of S100a9 -/- mice were established. The S100a9 -/- wild-type and mutant alleles were distinguished both by Southern hybridization with probe E following BglII digestion of mouse tail DNA and by a PCR-based assay. Interbreeding of S100a9 heterozygotes yielded the proportions of 1.0:1.9:1.0 for wild-typeheterogeneousnull mice, approximating the expected ratio for Mendelian inheritance.
  • Phenotype: No phenotype as yet
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Description: S100a9 and its heterodimeric partner, S100a8, are a cytosolic calcium-binding protein, highly expressed in neutrophils and monocytes. This knockout mice generated by targeted disruption of the gene is useful for understanding the function of S100a9 and the dispensability of both S100a8 and S100a9 for myeloid cell functions.
  • Research area: Cancer; Cell biology; Genetics; Immunology
  • Production details: The S100a9 gene was disrupted in ES cells with a replacement-type targeting vector and the strategy shown in the publication. ES cells were electroporated, and of 196 neomycin (G418)-resistant clones, 13 were correctly targeted. Two targeted clones were injected into C57BL/6 mouse blastocysts, and two independent strains of S100a9 -/- mice were established. The S100a9 -/- wild-type and mutant alleles were distinguished both by Southern hybridization with probe E following BglII digestion of mouse tail DNA and by a PCR-based assay. Interbreeding of S100a9 heterozygotes yielded the proportions of 1.0:1.9:1.0 for wild-type–heterogeneous–null mice, approximating the expected ratio for Mendelian inheritance.

  • For Research Use Only

Target Details

  • Target: S100a9

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  De Filippo et al. 2014. FASEB J. 28(8):3600-8. PMID: 24776746.
  • •  McNeill et al. 2014. Int J Cancer. 135(4):798-808. PMID: 24436096.
  • •  A new protective role for S100A9 in regulation of neutrophil recruitment during invasive pneumococcal pneumonia.
  • •  S100A9 has a protective role in inflammation-induced skin carcinogenesis.
  • •  Croce et al. 2009. Circulation. 120(5):427-36. PMID: 19620505.
  • •  Myeloid-related protein-8/14 is critical for the biological response to vascular injury.
  • •  Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
  • •  Myeloid cell function in MRP-14 (S100A9) null mice.