EXPERIMENTAL MODELS
Contributor Information
- Name Nancy Hogg
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: LFA1 -/- Mouse
- Alternate names: LFA-1, Integrin beta-2, Complement receptor C3 subunit beta, CD18
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Inflammatory disease;
- Model: Knock-Out
- Conditional: No
- Genetic background and cross history: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.
- Strain: C57BL/6
- Description: In vivo study of LFA-1 knockout; in vivo study of inflammation and leukocyte recruitment;
- Research area: Genetics; Immunology
- Production details: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.
- For Research Use Only
Target Details
- Target: Lymphocyte Function-associated Antigen-1 (LFA1)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
References
- • Henderson et al. 2001. J Exp Med. 194(2):219-26. PMID: 11457896.
- • The use of lymphocyte function-associated antigen (LFA)-1-deficient mice to determine the role of LFA-1, Mac-1, and alpha4 integrin in the inflammatory response of neutrophils.
- • Berlin-Rufenach et al. 1999. J Exp Med. 189(9):1467-78. PMID: 10224287.
- • Lymphocyte migration in lymphocyte function-associated antigen (LFA)-1-deficient mice.
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