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Contributor Information

  • Name Christoper Stipp
  • Institute University Of Iowa Research Foundation
  • Primary citation Zhu et al. 2022. npj Precis. Onc. PMID: 36271142

Tool Details

  • Tool name: vRRP3 cell line
  • Alternate names: vRRP3
  • Tool type: Cell Lines
  • Parental cell line: A375 Melanoma (ATCC)
  • Organism: Human
  • Donor: A375 (ATCC) -human melanoma cell line of a solid tumor from a 54-year-old female.
  • Tissue: Skin
  • Gender: Female
  • Cancer type: Skin cancer
  • Morphology: Diffuse Morphology
  • Description: Drug-resistant sub-lines of A375 that are BRAF^V600E and NRAS WT. VRPP3 Harbours RAC1^N921 but does not harbour NRAS mutations or BRAF genomic alterations, which drive drug resistance through MAPK-reactivation. It is a VEM-resistant derivative of A375. vRPP3 served as a positive control as it harbours a heterozygous N92I mutation in RAC1, con?rmed via Sanger sequencing. Shows Increased VAV1 protein levels. Showed decreased MEK Phosphorylation on S217(Raf Site) and Increased MEK Phosphorylation on S298 (Pak Site).
  • Research area: Cancer; Drug development
  • Production details: Spontaneous vemurafenib-resistant clones and populations were created after 2–3 and 4–6 weeks cultured in 3 mm vemurafenib. maintained in media with 3 mm vemurafenib.

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: DMEM(Gibco) suplemented with penicillin/streptomycin (Gibco) and 10% FBS (Gibco).

Documentation

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References

  • •  Feddersen et al.2019. Cancer Res. 79(19).PMID: 31416844