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Contributor Information

  • Name Peter Flatt and Neville McClenaghan
  • Institute Ulster University
  • Primary citation McClenaghan NH etal. 1996. J Endocrinol.;148(3):409-17 PMID: 8778219

Tool Details

  • Tool name: BRIN-BG7 cell line
  • Tool type: Cell Line
  • Tool sub-type: Hybrid cell line
  • Parental cell line: RINm5F cell line
  • Organism: Rat
  • Tissue: Pancreas
  • Disease: Diabetes
  • Morphology: Epitheloid
  • Growth properties: Adherent
  • Model: Transgenic
  • Model description: Hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma)
  • CRISPR: No
  • Application: producing pure insulin secreting cells when stimulated
  • Description: A hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic beta cell function as they showed insulin output 5–10 times greater than parent RINm5F cells
  • Research area: Disease (not cancer)

  • For Research Use Only

Target Details

Application Details

  • Application: producing pure insulin secreting cells when stimulated

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
  • Temperature: 37°C
  • Atmosphere: 5% CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry Ice
  • Initial handling information: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cmÂ? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cmÂ? can be expected.
  • Mycoplasma free: Yes

Documentation

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References

  • •  McClenaghan NH et al. 1999. J Mol Med. 77(1):235-43 PMID: 9930971.