CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-BRAFm-6 cell line
- Alternate names: 06M02007
- Clone: BRAF_type-06
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type: Type-06; Type mutation: D594G; CDS mutation: c.1781A>G; AA mutation: p.D594G; COSMIC Mutation ID: COSM467
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. BRAF (B-Raf proto-oncogene, serine/threonine kinase). BRAF belongs to the serine threonine protein kinase and is said to activate the MAPK pathway by receiving a signal from the activated RAS in the cell proliferation signaling pathway. Mutations of the BRAF gene are found in malignant melanoma, colon and rectal cancer, and chronic lymphocytic leukemia and is said to cause excessive signal transmission to disable the regulation of cell proliferation. Mutations of V600E are known as somatic cell mutations that are often found in cancer.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: B-Raf proto-oncogene, serine/threonine kinase [BRAF]
- Target background: Gene ID: 673; References: DNA (mRNA): NM_004333.4; Protein: NP_004324.2
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5%Â CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
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