CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941
Tool Details
- Tool name: TOV-3041G cell line
- Alternate names: TOV-3041G
- Tool type: Cell Lines
- Organism: Human
- Donor: Age of diagnosis: 61; BRCA hereditary status: negative Grade 3 Stage IVA; Mutations: P53 negative; Carboplatin/Taxol
- Tissue: Derived from high-grade serous tumors
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: Small epithelial type cells and consistently formed compact spheroids
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epithelial ovarian cancer cell lines spontaneously derived from high-grade serious tumors of patient who was treated with chemotherapy before collection due to recurring disease. Cell line was derived from rare case of HGS EOC where a somatic TP53 mutation was not detected by mutation screening. However, the absense of p53 mRNA and protein in addition to impaired p53 function was detected in this line. Therefore, this cell line has features characteristic of a p53-null mutation. It is suggested that in this cell line, TP53 regulation occurs at the mRNA level rather than protein level.
- Research area: Cancer
- Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly.
- Additional notes: Patient 3041 was age 61 at diagnosis, BRCA hereditary status was negative, and disease stage was IVA. First line treatment was carboplatin/taxol, then sugery, followed by Cisplatin/taxol. Patient had no prior personal history of cancer but was part of a breast cancer prevention trial of tamoxifen previously. Year of sampling was 2007.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
References
- • Vias et al. 2023. Elife. 11
- • 12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16
- • 14(22):5628. PMID: 36428724 Asare-Werehene et al. 2023. Cancers (Basel). 30
- • 15(9):2566. PMID: 37174032
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