CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-KRASm-b3 cell line
- Alternate names: 06M00006
- Clone: KRAS-b_type-03
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type: Type-03; Type mutation: G13D; CDS mutation: c.38G>A; AA mutation: p.G13D; COSMIC Mutation ID: COSM532
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. KRAS (Kirsten rat sarcoma viral oncogene homolog).KRAS is known as a gene involved in the development and progression of cancer by regulating proteins involved in survival of cells and angiogenesis at the downstream signaling pathway of epidermal growth factor receptor (EGFR) which is known as the switch of the proliferation of cancer cells. Mutations of the KRAS gene are found in various cancers such as rectal and lung cancer, and it is regarded to cause proliferation of cancer cells constantly, even without being activated by the signaling pathway through EGFR. Mutations of G12, G13 and Q61 are known as somatic cell mutations often found in cancer.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: KRAS proto-oncogene, GTPase [KRAS], Kirsten rat sarcoma viral oncogene homolog
- Target background: Gene ID: 3845; References: DNA (mRNA): NM_004985.3; Protein: NP_004976.2
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5%Â CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
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