CELL LINES
Contributor Information
- Name Julia Reichelt
- Institute Newcastle University
Tool Details
- Tool name: K38 Keratinocyte Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Disease: Dermatology
- Growth properties: Adherent
- Model: Primary line
- Description: The K38 Keratinocyte Cell Line has been established from the skin of neonatal WT mice. The prospective commercial application of the line is in the generation of in vitro epidermal ÄË?Â?Âskin equivalentsÄË?Â? to be used as screening tools to look at the activity of NCEÄË?Â?Âs and/or NBEÄË?Â?Âs of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments Murine keratinocyte stem cell line
- Research area: Drug development
- Production details: Keratinocytes were isolated from neonatal wild-type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
- Cellosaurus ID: CVCL_W141
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: The cell line grown is low calcium medium, Ham's F12/DMEM with 10% FCS and several supplements at 32?°C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 ??g/ml hydrocortisone, 5 ??g/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM glutamine, 1 mM
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Reichelt and Haase, 2010. Methods Mol Biol. 585:59-69. PMID: 19907996 HÄ?her T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602
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