CELL LINES
Contributor Information
- Name Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath
- Institute The Centre for Cellular and Molecular Platforms C-CAMP
Tool Details
- Tool name: Serum Free-Suspension adapted recombinant CHO-K1 cell line with stable production of second generation Human Erythropoietin protein called Darbepoetin (Darbe)
- Alternate names: haematopoietin, or haemopoietin
- Tool type: Cell Lines
- Parental cell line: CHO-K1
- Organism: Hamster
- Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbepoetin gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The Darbepoetin is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.
- Tissue: Ovary
- Disease: Therapeutics; Haematopoiesis; Anaemia
- Conditional: No
- Research area: Drug development
- Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbepoetin gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The Darbepoetin is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.
- For Research Use Only
Target Details
- Target: Erythropoietin (EPO), Darbepoetin (Darbe)
Application Details
Handling
- Growth medium: Grown in Serum Free CD Forti media. The cells are grown in a CO2 incubator at 37?°C with 5% CO2 with shaking.
- Temperature: 37C
- Atmosphere: 5% CO2
Documentation
Related Tools
References
- • A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)
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