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Contributor Information

  • Name Maria Carmo-Fonseca ; Sim?„ƒo Teixeira da Rocha
  • Institute Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes

Tool Details

  • Tool name: BRCA2 c.156_157insAlu iPSC line (2041) cell line
  • Tool type: Cell Lines
  • Parental cell line: Human fibroblasts
  • Organism: Human
  • Tissue: Fibroblast
  • Disease: Hereditary breast cancer; Hereditary ovarian cancer
  • Model: Cancer Model
  • Conditional: No
  • Description: BRCA2-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA2-associated tumors, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA2 mutant hereditary cancers.
  • Research area: Cancer; Genetics; Stem cell biology
  • Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy a female donor heterozygous for the BRCA2 mutation c.156_157insAlu.

  • For Research Use Only

Target Details

  • Target: Hereditary breast and ovarian cancer

Application Details

Handling

  • Format: Frozen
  • Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3â?‚€?‚“4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 â?‚—ĚšC in a humidified 5% CO2 incubator.
  • Shipping conditions: Dry ice

Documentation

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