CELL LINES
Contributor Information
- Name Thomas Reid
- Institute National Cancer Institute (NCI)
- Primary citation Padilla-Nash et al. 2012. Genes Chrom Cancer. 51(4):353-374. PMID: 22161874.
Tool Details
- Tool name: NCI-HPN-F43-Mam Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Epithelial
- Gender: Female
- Cancer type: Breast cancer
- Disease: Cancer
- CRISPR: No
- Application: Identification of cancer-specific genes & signalling pathways
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Description: NCI-HPN-F43-Mam is produced from spontaneously transformed primary cell cultures without genetic, viral or chemical manipulation. This murine cell line is derived from normal epithelial cells in the mammary gland.
Previous applications of this cell line include telomerase analysis by fluorescence in situ hybridisation (FISH).
This in vitro model allows study of sequential genetic and genomic alterations in the natural process of oncogenesis. - Research area: Cancer
- For Research Use Only
Target Details
Application Details
- Application: Identification of cancer-specific genes & signalling pathways
Handling
- Growth medium: DMEM/F12 media without phenol-red indicator (Invitrogen)
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Padilla-Nash et al. 2012. Genes Chrom Cancer. 51(4):353-374. PMID: 22161874.
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