CELL LINES
Contributor Information
- Name Peter Bradding ; Harvinder Virk
- Institute University of Leicester
Tool Details
- Tool name: Transient receptor potential ankyrin 1 (TRPA1)-GFP HeLa cell line
- Tool type: Cell Lines
- Parental cell line: HeLa
- Conditional: No
- Description: The transient receptor potential cation channel family member ankyrin 1 (TRPA1) is an ion channel with high Ca2+ permeability that is activated by numerous noxious stimuli and by multiple products of oxidative stress. TRPA1 is a drug target with antagonists in phase I and II clinical trials. It is considered a potential target in multiple pain conditions including neuropathic, inflammatory and migraine pain, in addition to cough sensitivity, airway inflammation and fibrosis This HeLa cell line is stably transfected with a dual-gene promotor (pCDH-CMV-MCS-EF1-copGFP) to express both GFP and hTRPA1. Forward TRPA1 Xba1 gggtctagaATGAAGCGCAGCCTGAGGAAG Reverse TRPA1 NotI ggggcggccgcttaAGGCTCAAGATGGTGTGTTTTTGCC
- Research area: Neurobiology
- For Research Use Only
Target Details
- Target: human Transient receptor potential cation channel family member ankyrin 1 (hTRPA1)
Application Details
Handling
- Format: Frozen
- Shipping conditions: Dry ice
Documentation
.svg)
Unit size: 
.svg)
.svg)
