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Contributor Information

  • Name Irene Leigh
  • Institute Queen Mary University of London

Tool Details

  • Tool name: IC1 SCC Cell Line
  • Alternate names: SCC, Squamous cell carcinoma, IC1
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Skin
  • Cancer type: Skin cancer; Skin cancer
  • Disease: Cancer
  • Model: Tumour line
  • CRISPR: No
  • Conditional: No
  • Description: IC1 squamous cell carcinoma cell line derived from a primary tumour. Background and Research Application The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process. This cell line has been used in a variety of research, including research in the role of Notch signalling mutations in SCC.
  • Research area: Cancer; Genetics
  • Production details: Cutaneous SCC cell line from 77 year old male. Moderately defined primary tumour from the right temple with germline DNA from lymphocytes. Patient was immuno-competent and did not receive immune therapy.
  • Additional notes: IC1 and IC1MET SCC Cell Lines cells are the most successful in xenografts and surface xenotransplantation (unpublished)

  • For Research Use Only

Target Details

  • Target: Squamous Cell Carcinoma

Application Details

Handling

  • Format: Frozen
  • Growth medium: Keratinocyte medium: 3:1 V/V mixture of DMEM and Ham’s F12 supplemented with 10% FBS and a cocktail of mitogens: 0.5 ug/ml hydrocortisone; 1x10-10M cholera toxin; 1.8 x 10-4 M adenine; 2 x 10–11 M liothyronine, 13 ng/ml; 5 μg/mL insulin; 10 ng/mL epidermal growth factor (EGF). FBS (10%) is routinely batch tested for enabling optimal clonal growth of cells. Culture SCC cell lines in the presence of mitotically inactivated 3T3-J2 feeder layers, which enhance proliferative capacity and reduces selective pressure on the cancer cells.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Characterisation tests: Genetic analysis [PMID: 30202019]
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Hassan et al. 2019. Int J Mol Sci. 20(14):3428. PMID: 31336867.
  • •  Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019.
  • •  McHugh et al. 2018. Oncotarget. 9(29):20265-20281. PMID: 29755650.
  • •  South et al. 2014. J Invest Dermatol. 134(10):2630-2638. PMID: 24662767.
  • •  Watt et al. 2011. Oncogene. 30(46):4666-77. PMID: 21602893.
  • •  Wang et al. 2011. Proc Natl Acad Sci U S A. 108(43):17761-6. PMID: 22006338.