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Contributor Information

  • Name Leonard Franks
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
  • Primary citation Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-23. PMID: 107359

Tool Details

  • Tool name: MB49 Cell Line
  • Alternate names: MB49, MB-49, MB49 Mouse Bladder Carcinoma Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Bladder
  • Gender: Male
  • Cancer type: Genitourinary cancer; Urinary bladder neoplasms; Bladder carcinoma; Mouse bladder transitional cell carcinoma
  • Morphology: A spindle-like epithelial morphology, or rounded
  • Growth properties: The cells do not form a 100% confluent monolayer but at 70% confluence tend to detach in small clumps that float in the media. About 10-20% of the cells will be attached with a spindle-like epithelial morphology, the remainder will appear rounded
  • Model: Tumourigenic line
  • Model description: The cells transplanted into syngeneic mice were shown to generate carcinomas
  • CRISPR: No
  • Conditional: No
  • Application: In vitro and in vivo model of bladder cancer; Metastatic urothelial carcinoma research
  • Description: MB49 cell line is an in vitro and in vivo model of bladder cancer, with enhanced metastatic potential for further migratory investigation.
    MB49 cells are a urothelial carcinoma line derived from an adult C57BL/Icrf-a’ mouse by exposure of primary bladder epithelial cell explants to 7,12-dimethylbenz[a]anthracene (DMBA). The syngeneic, murine model of bladder cancer has been widely used for more than 35 years. MB49 cell line was created to demonstrate the effects of ageing on neoplastic transformation in long term primary cultures of the bladder. There is a high age-associated bladder tumour incidence in men.
    The donor mouse was male, however, a 2012 karyotypic analysis [PMID: 22559978] found that the MB49 tumour cell line has lost the Y-chromosome and therefore does not express male-specific antigens. The loss of Y chromosome and lack of expression of male specific antigens is a frequent early event within bladder cancer, and thus it is an appropriate and effective model system.
    A more recent study [PMID: 26998503] evaluated the cell line’s sensitivity to testosterone and the pregnancy hormone, human chorionic gonadotropin (hCG). It was discovered that MB49 tumour growth was significantly greater in male mice than female mice and that pregnancy did not affect the size or rate MB49 tumour growth in female mice. MB49 cells failed to proliferate in response to hCG in vitro and the functional receptor for gonadotropins was absent. Dihydrotestosterone strongly stimulated growth of MB49 cells in vitro.
    MB49 cell line displays little to no MHC class I and II molecules but MHC class II antigens are produced when stimulated with IFN-y [PMID: 1638541].
    Implanted long term MB49 cells were subcutaneously injected into mice, with the primary tumours resected, mechanically disrupted and trypsin digested to obtain single cells, most of which were adherent but a small population of spheroidal 3D structures in culture. These are likely stem like, basal and highly metastatic, which is ideal for researching metastatic urothelial carcinoma.
  • Research area: Cancer
  • Production details: Derived from adult C57BL/Icrf-a’ mouse bladder epithelial cells via single 24 h treatment with 7,12-dimethylbenz[a]anthracene (DMBA) on the second day of primary culture
  • Cellosaurus ID: CVCL_7076

  • For Research Use Only

Target Details

Application Details

  • Application: In vitro and in vivo model of bladder cancer; Metastatic urothelial carcinoma research

Handling

  • Format: Frozen
  • Growth medium: DMEM Complete Medium (Sigma, Cat. No. SLM-241-B) or in DMEM-High Glucose (Sigma, Cat. No. D5796) with 10% FBS and 1X Penicillin/Streptomycin (optional)
  • Temperature: 37C
  • Atmosphere: 5%CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Cultured in antibiotics?: Penicillin/Streptomycin (optional)
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Albertó et al. 2019. Oncol Lett. 17(3):3141-3150. PMID: 30867744
  • •  Plote et al. 2019. Oncoimmunology. 8(5):e1577125. PMID: 31069136
  • •  Shi et al. 2019. Onco Targets Ther. 12:4403-4413. PMID: 31239709
  • •  White-Gilbertson et al. 2016. Bladder (San Franc). 3(1):. PMID: 26998503
  • •  Kasman et al. 2013. J Vis Exp. (82):50181. PMID: 24326612
  • •  Zhu et al. 2013. BMC Urol. 13:57. PMID: 24188098
  • •  Fabris et al. 2012. Cancer Genet. 205(4):168-76. PMID: 22559978
  • •  Chen et al. 2009. J Urol. 182(6):2932-7. PMID: 19853870
  • •  Loskog et al. 2005. Lab Anim. 39(4):384-93. PMID: 16197705
  • •  Brocks et al. 2005. J Urol. 174(3):1115-8. PMID: 16094076
  • •  Günther et al. 1999. Cancer Res. 59(12):2834-7. PMID: 10383142
  • •  Lattime et.al. 1992. Cancer Res. 52(15):4286-90. PMID: 1638541
  • •  Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-23. PMID: 107359